Anesthesiology
Pegah Parhizkar; Rahim Mohammadi; Seyed Mohammad Hashami-Asl; Ghader Jalilzadeh-Amin; Farshid Sarrafzadeh-Rezaei
Volume 15, Issue 1 , January 2024, , Pages 21-26
Abstract
The aim of this study was to compare the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses. Four healthy cross-bred horses were included in the study. They were assigned to two treatments. In treatment I (T1), the animals received ...
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The aim of this study was to compare the sedative and cardiovascular effects of the combination of acepromazine-clonidine versus acepromazine-xylazine in horses. Four healthy cross-bred horses were included in the study. They were assigned to two treatments. In treatment I (T1), the animals received xylazine hydrochloride (1.00 mg kg-1) in combination with acepromazine maleate (0.05 mg kg-1) intravenously (IV). In treatment II (T2), the animals received intra-gastric administration of clonidine (0.002 mg kg-1) followed by acepromazine (0.05 mg kg-1; IV) after 60 min. Head height above the ground (HHAG) and echocardiographic indices were evaluated. In T1, recordings were made 5 min before and 5, 15, 30, 60, and 90 min after drug administration. In T2, recordings were made 5 min before clonidine, 55 min after clonidine administration, and then 5, 15, 30, 60, and 90 min after acepromazine injection. Analyses of the data showed there were not significant differences regarding HHAG and echo-cardiographic indices between two treatments. For sedation of healthy horses, it was concluded that intra-gastric administration of clonidine and IV administration of acepromazine showed similar sedative and cardiovascular effects compared to IV acepromazine-xylazine administration.
Surgery
Emad Vakili-Sadeghi; Alireza Najafpour; Rahim Mohammadi
Volume 14, Issue 7 , July 2023, , Pages 389-395
Abstract
The torsion model of testis in a rat was adopted for evaluation of possible effects of propolis (Prop) on ischemia-reperfusion (IS/REP) injury. The healthy male Wistar rats (totally 24 animals) were randomized into four groups (n = 6) and animals experienced bilateral testicular torsions as follows: ...
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The torsion model of testis in a rat was adopted for evaluation of possible effects of propolis (Prop) on ischemia-reperfusion (IS/REP) injury. The healthy male Wistar rats (totally 24 animals) were randomized into four groups (n = 6) and animals experienced bilateral testicular torsions as follows: In sham group just, laparotomy was performed and in IS group, animals experienced a 3 hr period testicular IS. In IS/REP group, a 3 hr period of IS followed by a 3 hr period of testicular REP for left testis and a one-week testicular REP for right testis were done. In this group animals were gavaged by 1.00 mL normal saline 1 hr before the onset of IS. In IS/REP/ Prop group, the same procedures for IS/REP animals were followed as well as gavage of 1.00 mL Prop extract solution 1 hr before the onset of IS. Analyses of biochemistry, histology, inflammatory biomarkers and sperm parameters were carried out. In IS/REP/Prop group, nitric oxide synthase malondialdehyde, myeloperoxidase and 8-hydroxy-2 deoxyguanine in IS/REP/Prop group were significantly decreased and, superoxide dismutase, total glutathione, glutathione peroxidase, glutathione reductase and glutathione S-transferase were significantly increased compared to the other animals. In IS/REP/Prop group, seminiferous tubules (with normal spermatogenesis) showed all stages of spermatogenic cells with plentiful spermatozoa. Tubular deterioration and atrophy and spermatogenic cell loss in were seen in a limited extent. The mean concentrations of Interleukin-1 beta and tumor necrosis factor alpha in IS/REP/Prop were significantly decreased. Sperm quality was significantly improved by Prop in IS/REP/Prop group. It was concluded that Prop could be supportive in diminishing IS/REP injury in testicular tissue exposed to ischemia.
Histology
Saeede Amani; Rasoul Shahrooz; Esmaeil Mortaz; Rahim Hobbenaghi; Rahim Mohammadi; Ali Baradar Khoshfetrat
Volume 10, Issue 1 , March 2019, , Pages 23-30
Abstract
The aim of this study was to find a proper method for improvement of ischemic condition in the rat hind limb and also to observe the efficacy of cell engraftment with alginate/gelatin three-dimensional scaffolds. Eighteen male Wistar rats weighing 200 to 250 g were randomly divided into three groups ...
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The aim of this study was to find a proper method for improvement of ischemic condition in the rat hind limb and also to observe the efficacy of cell engraftment with alginate/gelatin three-dimensional scaffolds. Eighteen male Wistar rats weighing 200 to 250 g were randomly divided into three groups (n = 6) including a) ischemia group; in which femoral artery was removed after ligation at the distance of 5 mm, b) scaffold group; in which hydrogel scaffold was added to the site of transected femoral artery and c) test group; in which in addition to hydrogel scaffold, mast cells (MCs) were also added (1 × 106 cells). Analysis of capillary density, artery diameter, histomorphometric parameters and immunohistochemistry in transected location were done on day 14 after femoral artery transection. The average number of blood capillary was significantly higher in the test group than other groups. Also, the average number of medium and large blood vessels was significantly higher in the test group compared to ischemia and scaffold groups. Application of MCs through the use of hydrogel scaffolds (alginate/gelatin) can be considered as a new approach in the application of stem cells for therapeutic angiogenesis under ischemic conditions which can improve the angiogenesis process in patients with peripheral artery diseases.
Saeed Azizi; Rahim Mohammadi; Keyvan Amini; Roza Fallah; Kiana Karegar
Volume 1, Issue 2 , September 2010, , Pages 107-115
Abstract
Despite promising results observed using silicone rubber chamber, no previous comprehensive work was performed on behavior of the conduit. Present study aimed at further functional, histomorphometrical and immunohistochemical assessment of nerve regeneration in the same animal along a 10-mm rat ...
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Despite promising results observed using silicone rubber chamber, no previous comprehensive work was performed on behavior of the conduit. Present study aimed at further functional, histomorphometrical and immunohistochemical assessment of nerve regeneration in the same animal along a 10-mm rat sciatic nerve gap. Fifty- four male Wistar rats were divided into three experimental groups (n = 18), randomly: Sham-operation (NC), Transected control (TC) and silicone conduit (SIL). In NC group after anesthesia left sciatic nerve was exposed through a gluteal muscle incision and after haemoestasis the muscle was sutured. In TC group left sciatic nerve was exposed the same way and transected proximal to the tibio-peroneal bifurcation leaving a 10-mm gap. In SIL group left sciatic nerve was transected the same way and proximal and distal stumps were each inserted into a silicone tube. Each group was subdivided into three subgroups of six animals each and were studied 4, 8, 12 weeks after surgery. Functional analysis showed significant improvement of nerve function in SIL group than in TC group (P < 0.05). Morphometric indices and immuohistochemistry indicated there were significant differences (P < 0.05) between SIL and TC groups 12 weeks after surgery. Silicone entubulation technique has offered the hope of providing a method for achieving the peripheral nerve regeneration in the least harmful way that is available, easily performed. Using silicone tubes in bridging of nerve defects could be promising because it is inert and does not induce extensive scarring or degeneration after implantation.
Anesthesiology
Farshid Sarrafzadeh-Rezaei; Siamak Asri-Rezaei; Mojtaba Hadian; Rahim Mohammadi; Maryam Asfari
Volume 1, Issue 1 , June 2010, , Pages 7-11
Abstract
Rabbits are widely used as laboratory animals for experimental surgery. Anesthesia of rabbits may present complications unless the method is easy to apply and safe to use. In present study, effects of different dosages of vitamin C on thiopental sodium induced anesthesia in 25 male New Zealand white ...
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Rabbits are widely used as laboratory animals for experimental surgery. Anesthesia of rabbits may present complications unless the method is easy to apply and safe to use. In present study, effects of different dosages of vitamin C on thiopental sodium induced anesthesia in 25 male New Zealand white rabbits were studied. In the animals that had not received vitamin C treatment before thiopental sodium induced general anesthesia, return mean time of front limb pedal, corneal and ear pinch reflexes were 6.40 ± 1.67, 6.60 ± 2.96 and 8.00 ± 2.58 minutes, respectively. Pre-treatment of rabbits with 30 and 240 mg kg-1 (IV) of vitamin C followed by thiopental sodium 20 mg kg-1 (IV) resulted in significant (P < 0.05) increase in front limb pedal reflex return mean time to 13.00 ± 2.24 and ear pinch to 11.60 ± 4.16 minutes, respectively. There was also significant (P < 0.05) decrease in the heart rate following induction of anesthesia in the animals pre-treated with 30 and 90 mg kg-1 (IV) vitamin C and no change in the animals pre-treated with 240 mg kg-1 (IV) vitamin C. Serum analysis indicated a significant (P < 0.05) increase in blood glucose. These results suggest that premedication of rabbits with vitamin C despite potentiating of thiopental sodium anesthesia in rabbits is not dose dependent.
